background: atherosclerosis, a chronic inflammatory disease of the vessel wall is characterized by local and systemic immune responses to a variety of antigens. oxidized low-density lipoprotein (oxldl) is considered as an important determining factor in the pathogenesis of atherosclerosis. objective: the purpose of this study was to investigate the degree of peripheral blood mononuclear cells (...

introduction: multiple sclerosis (ms) is generally known as a manageable but not yet curable autoimmune disease affecting central nervous system. a potential therapeutic approach should possess several properties: prevent immune system from damaging the brain and spinal cord, promote differentiation of oligodendrocyte progenitor cells (opcs) into mature oligodendrocytes to produce myelin, preve...

objective: stevioside is a natural non-caloric sweetener which has been reported to have anti-inflammatory activity.  the aim of  the present study was to examine in vitro and in vivo effects of  stevioside on rats  plasma levels of tumor necrosis factor- α (tnf-α),  interleukin-1β (il-1β), tnf-α and il-1β release from lipopolysaccharide(lps)-stimulated rat peripheral blood mononuclear cells (p...

background , despite numerous studies over a decade, it still remains controversial about the biological effects of rf emf emitted by mobile phone telephony. objective , here we investigated the effect of 900 mhz gsm on the induction of oxidative stress and the level of intracellular reactive oxygen species (ros) in human mononuclear cells, monocytes and lymphocytes as defence system cells. met...

background: recognition of helminth-derived pathogen associated molecular patterns (pamps) by pattern recognition receptors (prrs), including toll like recep­tors (tlrs) is the first step towards initiating anti–helminth immune re­sponses. methods : using somatic antigens of ostertagia occidentalis, an important abomasal parasite of ruminants, the expression of ovine tlr2 and tlr4 in peripheral...

Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...

atherosclerosis is a chronic inflammatory condition that affects the arterial wall. oxidized low- density lipoprotein (ox-ldl) seems to have an important role in atherosclerotic plaque formation. this study was performed to investigate the effects of ox-ldl as well as pha on proliferation and gene expression of  peripheral blood  mononuclear  cells (pbmcs) in patients with atherosclerosis compa...

FRET based ratiometric switch for selective sensing of Al3+ with bio-imaging in human peripheral blood mononuclear cells (PBMCs).

cryopreservation is the method of choice for long term storage of human pbmcs. this study was designed to compare the different combinations of variables affecting the cryopreservation of pbmcs samples. the viability of pbmcs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with di...